INTRACELLULAR DISTRIBUTION OF CHOLINE OXIDASE

Intracellular distribution of choline oxidase.

Recently considerable attention has been centered upon the relation of choline oxidase activity to folic acid, the Leuconostoc citrovorum factor (LCF), ascorbic acid, and vitamin B,,. From this laboratory it has been indicated that rat liver choline oxidase can be stimulated in vitro under certain conditions by these factors, especially if the activity of the enzyme is previously decreased by d...

The distribution of choline oxidase activity in rat liver.

Rat liver was fractionated essentially as described by Hogeboom, Schneider, and Pallade (2). The liver was homogenized in 0.88 M sucrose (1 gm. of liver to 10 ml.) in the cold and centrifuged in 25 ml. Lustroid tubes in a 32” angle centrifuge (Custom Scientific Instruments, Inc.) in a cold room kept at 5”. The homogenate was separated into nuclear, mitochondrial, and supernatant fractions. The ...

Intracellular distribution of the lysyl oxidase propeptide in osteoblastic cells.

Lysyl oxidase plays a critical role in the formation of the extracellular matrix, and its activity is required for the normal maturation and cross-linking of collagen and elastin. An 18-kDa lysyl oxidase propeptide (LOPP) is generated from 50-kDa prolysyl oxidase by extracellular proteolytic cleavage during the biosynthesis of active 30-kDa lysyl oxidase enzyme. The fate and the functions of th...

A Novel Choline Biosensor Based on Immobilization of Enzyme Choline Oxidase on the β-Ga2O3 Nanowires Modified Working Electrode

This article is a report on a novel and high-stability biosensor with minor interference effects of surface modification of working electrode with β-Ga2O3 nanowires (NWs) on choline oxidase biosensor were investigated in an electrochemical detection system. β-Ga2O3 NWs were materialized on the silicon substrate in a catalyst-free growth mechanism. The β-Ga2O<sub...

Modeling Spatial Distribution of Intracellular Organelles by Molecular Motors